Product Name: Citrate Phosphate Buffer
Product Use: Laboratory reagent, pH control in biochemical experiments
Manufacturer: Details available on supplier’s product label, often sourced from established chemical companies
Emergency Phone Number: Supplied by distributor or national poison control center
Chemical Family: Buffer solution containing citric acid, sodium citrate, disodium hydrogen phosphate, and sodium dihydrogen phosphate
Molecular Formula: Mixture, main constituents: C6H8O7·Na3PO4
Synonyms: CPB, Citrate-Phosphate Buffer Solution
Physical State: Liquid or powder, based on preparation
Major Hazards: Classified as not hazardous by GHS for most laboratory concentrations; possible mild skin and eye irritant
Label Elements: Not classified as dangerous under EU CLP, no pictogram for most concentrations
Potential Health Effects: Eye and skin irritation if concentrated, may cause gastrointestinal upset if ingested in large quantities
Inhalation Risk: Dust from powder form may cause minor respiratory discomfort
Target Organs: Eyes, skin, respiratory tract
Citric Acid: CAS 77-92-9, 1–10% w/v, weak acid
Sodium Citrate: CAS 68-04-2, 1–10% w/v, buffer/chelating agent
Disodium Hydrogen Phosphate: CAS 7558-79-4, 1–10% w/v, buffer
Sodium Dihydrogen Phosphate: CAS 7558-80-7, 1–10% w/v, buffer
Water: CAS 7732-18-5, balance to 100%
Impurities: No hazardous impurities expected at relevant lab concentrations
Inhalation: Move to fresh air immediately; seek medical attention if symptoms persist
Skin Contact: Wash with soap and plenty of water; remove contaminated clothing
Eye Contact: Rinse gently with plenty of water for several minutes, remove contact lenses if present, continue rinsing
Ingestion: Rinse mouth with water, drink water if conscious, never give anything by mouth to an unconscious person, seek medical help if feeling unwell
Medical Notes: No specific antidote, treat symptomatically
Suitable Extinguishing Media: Use water spray, CO2, dry chemical, or foam
Special Hazards: Decomposition may produce sodium oxides or phosphorous oxides at extremely high temperature
Protective Equipment: Standard fire-fighting personal protection, including self-contained breathing apparatus
Fire-Fighting Instructions: Avoid inhalation of fumes, use water spray to cool unopened containers
Personal Precautions: Wear gloves and protective eyewear to minimize contact
Spill Cleanup Methods: Absorb with inert material (sand, absorbent pad), sweep up, place in a suitable closed container, rinse area with plenty of water
Environmental Precautions: Prevent entry to drains and natural waterways, notify authorities if large quantities enter environment
Disposal: Dispose in accordance with local regulations
Handling: Avoid dust or vapor formation, don’t breathe powder or mist, practice good laboratory hygiene
Storage Requirements: Store tightly sealed at room temperature, away from incompatible substances (strong acids or bases)
Storage Environment: Keep container dry and protected from direct sunlight
Special Considerations: Label containers clearly, keep separate from food and beverages
Engineering Controls: Work in a well-ventilated area or under fume hood
Personal Protective Equipment (PPE): Safety goggles, nitrile gloves, lab coat
Respiratory Protection: Not required for regular lab use; use NIOSH-approved respirator for large-scale powder handling
Hygiene Measures: Wash hands after use, remove contaminated clothing and wash before reuse
Exposure Limits: Not established for buffer constituents in typical lab concentrations
Physical State: Colorless to slightly yellow solution or white crystalline powder
Odor: Odorless
pH: 3.0–7.0, adjustable
Boiling Point: Approx. 100°C (solution)
Melting Point: Not applicable for solution; solid components melt above 150°C
Solubility: Readily soluble in water
Specific Gravity: Approx. 1.0 (solution)
Vapor Pressure: Not applicable
Flash Point: Not flammable
Decomposition Temperature: Above 200°C for solids
Appearance: Clear liquid or white crystalline powder
Chemical Stability: Stable under standard laboratory conditions
Conditions to Avoid: Excessive heat, direct sunlight, mixing with strong acids or strong bases
Incompatible Materials: Strong oxidizers, strong acids/bases
Hazardous Decomposition Products: May release sodium oxides or phosphorous oxides at high temperatures
Polymerization: Will not occur
Route of Entry: Ingestion, inhalation, skin or eye contact
Acute Effects: Mild eye, skin, and respiratory irritation possible; high ingestion may cause gastrointestinal upset
Chronic Effects: No reliable chronic toxicity data for mixture at typical concentrations
Carcinogenicity: Not listed as a carcinogen by IARC, NTP, or OSHA
Mutagenicity: No data suggesting mutagenic effects from buffer ingredients at laboratory exposures
LD50 (Estimated): Citric acid oral LD50 (rat): 3,000 mg/kg; sodium phosphate oral LD50 (rat): 17,000 mg/kg
Symptoms: Redness or irritation in eyes/skin, coughing on inhalation of dust
Aquatic Toxicity: Not expected to pose significant hazard at typical concentrations, large releases may cause minor shifts in water pH
Persistence/Degradability: Components degrade readily in environment, citric acid breaks down to carbon dioxide and water
Bioaccumulation Potential: Low, compounds are water soluble and do not bioaccumulate
Mobility in Soil: High, will leach through soil quickly
Other Adverse Effects: Avoid uncontrolled release to surface water due to potential pH disturbances
Waste Disposal Methods: Flush small quantities to approved wastewater system if allowed; dilute heavily with water; large volumes must comply with local, regional, and national regulations
Contaminated Packaging: Rinse thoroughly, dispose of as non-hazardous laboratory waste unless contamination is suspected
Special Considerations: Do not mix with other chemical wastes
Local Regulatory Requirements: Follow rules set by governing agency, such as EPA, city chemical waste regulations
UN Number: Not regulated for transport
UN Proper Shipping Name: Not dangerous goods
Transport Hazard Classes: No hazard class
Packing Group: Not applicable
Special Precautions: Avoid extreme temperatures, package to prevent leaks, ship in closed, labeled containers
Land, Sea, Air Transport: No restrictions for buffer concentrations typically used in labs
Global Inventories: Components listed in TSCA (USA), EINECS (EU), DSL (Canada)
OSHA Status: Not hazardous at typical concentrations
SARA Title III: Not subject to reporting
California Proposition 65: Not listed
REACH Status: Exempt or pre-registered
Labeling: No hazard symbol required for dilute solutions
Other Regulations: Use under general laboratory chemical safety guidelines, consult local laws for any additional requirements
